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Figure 1. A) Power density output curves for biofuel cells based on different bioanodes. (a) XDH/MWNTs/GCE bioanode, 10 mM NAD+; (b) XDH/MWNTs/GCE bioanode, 30 mM xylose + 10 mM NAD+; (c) XDH/PBCB/MWNTs/GCE bioanode, 10 mM NAD+; (d) XDH/PBCB/MWNTs/GCE bioanode, 30 mM xylose + 10 mM NAD+; (e) bacteria-XDH/PBCB/MWNTs/GCE bioanode, 30 mM xylose + 10 mM NAD+. B) Graph of the relation between the maximum output power density and the xylose concentration of a bio-anode fuel cell using bacteria-XDH/PBCB/MWNTs/GCE. The supporting electrolyte was an oxygen saturated 0.1 M PBS solution (pH 7.4).
Recently, under the support of projects supported by the National Natural Science Foundation of China and the Chinese Academy of Sciences’ Knowledge Innovation Project, the head of the biosensor team of the Institute of Bioenergy and Bioprocess Research of the Chinese Academy of Sciences and the 100-person plan selected by the Chinese Academy of Sciences, Liu Aixie, are based on xylose. Hydrogenase surface display system of microbial fuel cell research has made new progress.
Biofuel cells refer to the direct conversion of chemical energy from biofuels into electricity using microbes or enzymes as catalysts. Compared with the traditional fuel cell has the following characteristics: 1) a wide range of fuel sources, natural renewable organic matter may be used as fuel; 2) mild reaction conditions, can be carried out at room temperature, atmospheric pressure, neutral pH conditions; 3) biocompatible Good sex, can provide energy for artificial organs or biosensors implanted in the human body. The team constructed a microbiological plant for enzymes using bacterial surface display technology, eliminating the time consuming, costly purification process used in enzyme production (Analytical Chemistry 2012, 84, 275-282).
At the same time, the team constructed bacterial-XDH based on bacterial surface and optimized the conditions to construct a bacterial-XDH/polybright cresyl blue/multi-walled carbon nanotube/glassy carbon electrode (bacteria-XDH). /PBCB/MWNTs/GCE) Bio-anode and bilirubin oxidase-modified electrodes are bio-cathode membraneless biofuel cells. This system has an open circuit potential of up to 0.58 V and a maximum output power density of 63 μWcm-2 (Fig. 1, curve e). Compared to the same enzyme-purified XDH modified anode (Fig. 1, curve d), the power is increased by 60%. This research applied the bacterial surface display technology of enzymes to biofuel cells, which not only solved the problems of transmembrane electron transport and material transport that are common in microbial fuel cells, but also solved the low stability and cost of enzymes in enzyme fuel cell research. High question. (Biosensors & Bioelectronics 2013, 44, 160-163)
It is generally believed that 17% to 31% of lignocellulose will be converted into xylose after hydrolysis. How to increase the conversion rate of xylose from microbial fermentation has become one of the bottlenecks in the production of fuel ethanol from lignocellulose. At present, the microbial fermentation method takes a long time, has many by-products, and has a low yield, which reduces the utilization of xylose. This study is expected to open up a new way for the efficient use of lignocellulose hydrolysates, especially the direct transfer of lignocellulose hydrolysates into electrical energy.
In addition, based on the design and construction of a microbiological surface display system, the team developed a series of methods for the detection of xylose and glucose based on electrochemically modified electrodes. These methods enable highly sensitive, low-interference, rapid detection or co-detection of xylose or glucose in a complex system including lignocellulose degrading fluids. Relevant research results were published in Analytical Chemistry 2012, 84, 275-282 and Biosensors & Bioelectronics 2012, 33, 100-105; 2013, 42, 156-162; 2013, 45, 19-24.
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